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Fig. 2. Defects in secretory differentiation, but not vascular expansion, at day 15
of pregnancy. (A,B) Paraffin wax-embedded sections prepared from wild-type (A)
or Hif1a–/– (B) glands isolated at day 15 of
pregnancy stained with Hematoxylin and Eosin. Scale bar: 50 µm. Note the
striking block in differentiation in the
Hif1a–/– glands (B). (C,D) Patterning of the
vasculature in relationship to the mammary epithelium after lectin (green) and
phalloidin (red) staining in wild-type (C) and
Hif1a–/– glands (D).
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