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Fig. 9. Rescue of the LR defects of Inv/Inv embryos by artificial nodal flow. Both Inv/+ and Inv/Inv embryos were exposed to artificial leftward flow as described previously (Nonaka et al., 2002) and were then examined for the directions of heart looping and embryonic turning. (A) The numbers of embryos that exhibited normal or reversed heart looping or embryonic turning, or outlooping of the heart or an unturned tail were determined. (B,C) Examples of the rescued (B) and non-rescued (C) Inv/Inv embryos are shown. Heart looping is indicated by the broken lines. Scale bar: 1 mm.





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