Fig. 4. Expression of At.dpp transcripts in stage 5 embryos revealed by whole-mount in situ hybridization. (A,B) Early stage 5 embryo viewed from the top (A) and the lateral side (B) of the germ disc. The signal is seen as a spot at the center of the germ disc. The rim of the germ disc is marked by dots in B as well as in C and D. (C) Mid stage 5 embryo. A spot of signal is seen at an intermediate position between the center and the rim of the germ disc. (D) Late stage 5 embryo. A spot of signal is seen close to the rim of the germ disc. The spots of dpp expression correspond to the positions of the cumulus in the embryos. (E,F) Close-up of the cumulus in a stage 5 embryo. E focuses on the surface epithelial cell layer and F on the CM cells. The signal is seen in the CM cells (F) but not the surface epithelial cells (E). (G,H) Embryos at about 65 hours (G) and 75 hours (H) AEL. The strong signal of At.dpp is seen in developing limb buds (G) and the tip of the extending limbs (H). The ventral midline is weakly stained (H). Ch, chelicerae; Pp, pedipalp; L1-4, leg segments 1-4.

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