Fig. 7. Expression of At.otd and At.cad transcripts. Embryos stained by whole-mount in situ hybridization with the At.otd probe (A-D), At.otd and At.dpp mixed probes (E,F) or At.cad probe (G-J). (A,B) Stage 5 embryo viewed from the top (A) and the lateral side (B) of the germ disc. Expression of At.otd transcripts is observed in peripheral cells of the germ disc epithelium, three or four cells wide. (C) Stage 6 embryo viewed from the top of the germ disc. The pattern of At.otd expression is changed to an `open' circle. (D) Stage 7 embryo viewed laterally. Expression of At.otd is seen at the emerging anterior region of the embryo. (E,F) Late stage 5 (E) and early stage 6 (F) embryos. The pattern of the At.otd-positive cells becomes the `open' circle at almost the same time when the CM cells expressing At.dpp (arrowheads) reach the rim of the germ disc. (G,H) Stage 7 embryos viewed posteriorly (G) and laterally (H). Expression of At.cad transcripts is found at the most posterior region of the embryo corresponding to the caudal lobe. (I,J) High magnifications of the posterior end of a stage 7 embryo flat-mounted. I focuses on the surface of the embryo, and J on the inside. A pit is found at the center of the At.cad-expressing region (arrow in I). Below the pit, there is a population of cells expressing At.cad transcripts (arrow in J). Mesenchymal cells not expressing At.cad transcripts are also found. The margin of the forming germ band is indicated by dots in D and H. a, anterior; p, posterior; d, dorsal; v, ventral.

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