Fig. 3. Histological and morphological analysis of eye development in Mab21l1-deficient mice. Gross appearance of Mab21l1^+/- (A) and Mab21l1^-/- (B) eyes. The Mab21l1^+/- lens is well developed (A; P1, E16) and pigmented retinal epithelium is almost round, whereas the Mab21l1^-/- eye has no lens and the retinal pigmented epithelium is abnormally indented (B; P1, E16, E12.5). No difference is evident at E10.5. HE-stained coronal sections of E17 (C,D), E13 (E,F), E10.5 (G,H) and E9.5 (I,J) embryos. At E17 and E13, no lens is evident in the Mab21l1^-/- eye (D,F). Brackets (C,D) indicate the thickness of the retina, arrowheads (D) indicate abnormal corneal swelling and the arrow (D) indicates the optic nerve. (H) At E10.5, a malformed lens placode is evident in the Mab21l1^-/- eye (arrowheads). (I,J) At E9.5, little difference is evident between Mab21l1^+/- (I) and Mab21l1^-/- (J) eyes. The presumptive lens placode is abnormally thin and narrow in Mab21l1^-/- embryos (J; brackets) compared with Mab21l1^+/- embryos (I; brackets). Scale bars: 200 µm for C,D; 100 µm for E,F; 50 µm for G-J.

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