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Fig. 6. Analysis of cell proliferation and apoptotic cell death in
Mab21l1+/- and Mab21l1-/- lens
placodes. Immunohistochemical detection of BrdU incorporation in
Mab21l1+/- and Mab21l1-/- embryos at
various somite stages (A-H). Brackets (D,F) indicate reduced incorporation of
BrdU in Mab21l1-/- small lens placodes. Red dotted lines
indicate a Mab21l1+/- lens vesicle (G) and a
Mab21l1-/- malformed lens placode (H). The inset shows a
HE-stained serial section of the area within the box (H). TUNEL assay of
35-somite stage Mab21l1+/- (I) and
Mab21l1-/- (J) embryos. Numbers of TUNEL-positive cells
are slightly increased in the Mab21l1-/- lens pit
(arrowheads). Histogram showing the percentage of BrdU-positive cells in
Mab21l1+/- and Mab21l1-/- lens anlages
(K). The ratio of BrdU-positive cells is reduced in the
Mab21l1-/- mutants (t-test; 27 and 32-somite
stages, P<0.004; other somite stages, P<0.0001).
Vertical bars represent standard errors. lp, lens placode; lv, lens vesicle;
ov, optic vesicle; pi, lens pit; se, surface ectoderm. Scale bars: 50
µm.
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