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Supplemental Tables

This file contains the supplemental data referred to in the Materials and Methods section of the paper.

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Figure S1

The axons of dimm mutant neurons project normally. LK immunostaining in the peripheral projections of neuron A4 in dimm+/- (A) and dimm-/- (B) third instar stage larvae (n=9). Anterior is upwards and the ventral midline is towards the left. (C) The orientation of the LK-immunoreactive processes relative to muscle 8. Arrows indicate immunoreactive boutons. Scale bar: 50 mm.



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Figure S2

dimm controls levels of a constitutively secreted protein, CD8::GFP. (A) Ectopic expression of the CD8::GFP fusion protein (P{w+mC=UAS-mCD8::GFP.L}LL6) in the 34 Furin 1-positive neurons. In dimm-/- mutant CNS (third instar; R6, ap-Gal4/Rev8; UAS-CD8::GFP/+), all 34 Furin 1-positive neurons were visible, but some displayed reduced levels of CD8::GFP (A¢). (B) Mean pixel intensity (intensity index) for soma CD8::GFP fluorescence in four pairs of dorsal chain neurons (d5-d11; c929-positive) and two pairs of ventral chain neurons (v2a and v8; c929-negative) in six dimm+/- and 11 dimm-/- CNS. Because pixel intensities were measured using confocal z-series images (with a higher signal:background ratio), the intensity index scores were higher than in the preceding figures. *P<0.05; ***P<0.001. Scale bar: 50 mm.






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