Fig. 1. The c929 reporter gene is expressed in 200 peptidergic CNS neurons. (A) Confocal z-series of c929 reporter (c929-Gal4, P{UAS-lacZ}Bg4-1-2) expression in the larval CNS. Expression is detected in 200 neurons, in neuroendocrine release sites (asterisks) and in intrinsic cells of the endocrine corpora cardiaca (CC) (n>50). (B,B') Double-labeling for c929 reporter (B) and the peptide biosynthetic enzyme, PHM (B'). Yellow cells in the overlay (B'') were positive for both markers (n=8). (C) Double-labeling (C'') for c929 reporter (C; P{UAS-GFP}D1) (Yeh et al., 1995) and neuropeptides ending in the epitope, RF-amide (PT2 antiserum; RFa, C') (n=10). (D) Double-labeling (D'') for c929 reporter (D) and neuropeptides related to MM (D') (n=10). (E) Schematic illustration of the expression patterns for the c929 reporter, the PHM enzyme, and MM- or RFa-immunoreactive neuropeptides. Although generally c929 negative at this stage (mid-third larval instar), the MP1 and VA neurons express the c929 reporter gene for one or more brief periods during development. Scale bars: 50 µm.

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