Fig. 6. dimm^-/- mutant neurons differentiate, survive, and express non-secretory proteins at normal levels. (A) Thirty-four neurons (black circles) in the ventral nerve cord that co-express the c929 reporter, Furin 1 and ap-Gal4 (ap^Gal4). Additional ventral neurons (white circles) express ap-Gal4 but do not express the other two markers. (B) The dimm mutant phenotype for the 34 Furin 1 neurons. Reduction in Furin 1 immunostaining in the CNS of a third instar dimm^-/- (R6/Rev8) larva (B'), compared with a dimm^+/- (R6 or Rev8/+) sibling control (B) (n>50). Arrowheads indicate Tv and Tvb neurons. (C) Ectopic expression of the non-secretory fusion protein tau::Myc in the 34 Furin 1-positive neurons. In dimm^-/- mutant CNS (third instar; R6, ap-Gal4/Rev8; UAS-tau::Myc/+), all 34 Furin 1 neurons displayed anti-Myc immunostaining (C') that was comparable with that in the dimm^+/- sibling control (C; R6, ap-Gal4/+; UAS-tau::Myc/+). In both genotypes, each of the dorsal chain neurons (d1-d11) displayed projections that extended toward the midline (vertical arrows) and then ran longitudinally (horizontal arrows). We also observed normal immunostained projections in each abdominal nerve (arrowheads). (D) Mean pixel intensity (intensity index) for soma Furin 1 immunostaining in five pairs of dorsal chain neurons in seven dimm^+/- and 13 dimm^-/- CNSs. *P<0.05. Scale bars: 50 µm.

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