Fig. 5. Actin cones are stable structures. (A) A series of F-actin stainings after LTA treatments of different time duration. Even after 2 hours treatment, some actin cones remained visible. Scale bar: 10 µm. (B) Quantification of fluorescence intensity of individualization complex (IC; a cluster of 64 actin cones in a cystic bulge) after LTA treatment. In order to quantify the effect of treatments, fluorescence intensity of each IC was measured. In each of three experiments and at each time period of treatment, fluorescence intensities of 40 to 50 ICs were measured and average intensities were calculated.

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