Fig. 7. Ectopic lamellar-like structures on peripheral glia in Rac1L89 embryos do not always cover motor or sensory neuron projections. repo:GAL4 embryos carrying the UAS-actin-GFP marker and UAS-Rac1L89 transgenic construct are were double labeled for glial actin-GFP (green) and for motoneurons (A; red, mAb 1D4) and sensory neurons (B,C; red, mAb 22C10). Anterior is towards the top, CNS is towards the left. Stage 16 embryos are shown. (A) Lamellar-like structures of peripheral glia (arrows) do not overlap with motoneuron staining and are not found where sensory neurons normally occur. (B) Low magnification of Rac1L89 embryo with ectopic lamellar-like projections. The ectopic glial projections do not match sensory neuron patterning. (C) Higher magnification of box in B shows lamellar-like structures with fine spike-like actin-GFP formations further projecting out of the lamellae. The left lamellar-like structure appears to have a glial nucleus at its center. Peripheral glial nuclei are oval shaped and do not stain with Actin-GFP.

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