Fig. 4. In situ hybridization of ssdp probes to whole-mount embryos and dissected larvae. (A) Embryos at germband extension (stage 11 embryo, left) have widespread expression that increasingly becomes enriched in the developing central nervous system as germband retraction proceeds (stage 12 embryo, right). (B) At stage 13-14 of embryonic development, ssdp expression is largely restricted to the brain and ventral nerve cord. (C) Higher power ventral view of the ventral nerve cord showing pan-neuronal expression within the CNS. (D) In third instar larvae, ssdp transcripts are not detectable in the ventral nerve cord (arrowhead), but moderate ssdp expression was observed in the optic lobes of the brain hemispheres (arrows in D,E). (E,F) High levels of ssdp expression are observed in imaginal discs, including the anterior region of the antennal-eye disc (E, arrowhead) and uniform levels in the wing disc (F, left) and haltere disc (F, right).

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