Fig. 7. Mosaic analysis of ssdp^l(3)neo48 mutant clones reveals phenotypes similar to those of Chip and ap. Mounted wings of newly eclosed adults after heatshock-induced expression of FLP recombinase and subsequent recombination at FRT sites (see Materials and Methods). (A) A control clone along the native wing margin is marked by pwn. The border of the clone is outlined in red. Next to the clone the normal arrangement of the triple row of sensory bristles along the margin is shown, including a well-spaced row of dorsal chemosensory bristles (black arrow), a second and more tightly arrayed row of dorsal mechanosensory bristles (arrowhead), and a third row of ventral bristles (out of focal plane, gray arrow). Within the clone, bristles are mutant for pwn, but are otherwise normally specified, and there are no associated mutant phenotypes. (B) Low-power image of entire wing showing phenotypes associated with dorsal clones of ssdp^l(3)neo48 mutant cells in an individual in which clones were induced during second larval instar. Phenotypes include ectopic margin formation accompanied by ectopic wing outgrowth (arrow), and ectopic margins in the absence of outgrowth (arrowheads). Phenotypes were never associated with ventral ssdp^l(3)neo48 clones. (C) Higher power view of the outgrowth shown in B. The tip of the outgrowth (arrowhead) has ectopic sensory bristles that resemble the wing margin. (D) Close-up view of outgrowth tip shown in C. The ssdp^l(3)neo48 mutant clone (marked by pwn and outlined in red) lies near the tip of the outgrowth and is next to the ectopic margin. (E,F) Two focal planes of the same ectopic margin in the absence of wing outgrowth, the most common phenotype observed for ssdp^l(3)neo48 mutant clones. The extent of the ssdp^l(3)neo48 mutant clone on the dorsal surface of the wing is marked by pwn and outlined in red (E). The clone is near but not within the native wing margin, and results in the induction of ectopic bristles shown in the focal plane of F. (G) Loss of dorsal-specific bristles within an ssdp^l(3)neo48 mutant clone (outlined in red) that lies on the native wing margin. Outside the clone both the dorsal-specific chemosensory (black arrow) and mechanosensory (arrowhead) bristles are intact. Within the clone, however, these bristles are lost, despite the fact that the overall structure of the wing is undisturbed. The ventral specific bristles (gray arrow) lie outside the clone and remain intact. (H) A broad ssdp^l(3)neo48 mutant clone (outlined in red) that straddles the margin on both the dorsal and ventral surfaces results in complete loss of wing margin and some wing tissue, resulting in a nicked wing.

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