Fig. 3. hdl and tcf3b morpholinos (MOs) produce different phenotypes and ectopic expression of tcf3b can rescue the hdl MO phenotype. (A) The hdl and tcf3b MOs can specifically block translation of expression constructs in vitro, in a dose-dependent manner. The hdl MO has no effect on a tcf3b expression construct, and the tcf3b MO has no effect on a hdl construct. (B) Uninjected embryo at 72 hours post-fertilization (hpf). (C) Embryos injected with 1 ng of hdl MO have no eyes or telencephalon, similar to hdl mutant embryos. (D) Embryos injected with 1 ng of tcf3b MO have normal early brain patterning, but overall head size appears smaller (compare with B). (E) When 1 ng of both MOs are injected simultaneously, embryos develop with a much smaller head than with either MO alone (compare with C,D). (F) Rostral view of pax2.1 expression at 90% epiboly in an uninjected embryo, dorsal towards the right. (G) In hdl MO-injected embryos, pax2.1 expression is expanded rostrally. (H) When tcf3b mRNA is co-injected with the hdl MO, pax2.1 expression is restored to the normal domain of two stripes. (I) Normal morphology is observed at 24 hpf after co-injection of tcf3b mRNA and hdl MO. ov, otic vesicle.

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