Fig. 6. Zic1 represses Math1/Cath1 autoregulation. Chick neural tubes electroporated with Math1 (A) or Math1 plus ^mycZic1 (C) at HH14 and assayed at 24 hours for Cath1 expression by mRNA in situ hybridization. Arrows indicate the electroporated side. Math1 activates Cath1 expression (A) but this activity is inhibited by ^mycZic1 (C). Chick neural tubes co-electroporated with a Math1/lacZ transgene plus Math1 and Myc-tagged control vector (B), or Math1 and ^mycZic1 (D). Immunofluorescence of adjacent sections to detect ß-gal (B,D), Math1 (B',D'), Myc (B''), or Zic (D'') are shown. Note the dramatic activation of the Math1/lacZ transgene when co-electroporated with Math1 (compare Fig. 3E or 5C with B) and the block of this activation in the presence of ^mycZic1 (D).

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