Fig. 3. Expression of a neurone-specific marker in cells of proneural clusters in Notch, Su(H) and Psn wing imaginal discs of the late third larval instar stage. Anterior is towards the left, ventral towards the bottom. (A-C) Wing imaginal discs stained with anti 22C10 antibody. (D,E) Wing imaginal discs stained with anti Elav antibody. (A) Df(1)N^81K mutant clones in the notum of a wing imaginal disc revealed by the lack of the GFP marker. The arrows indicate proneural clusters where the cells express 22C10. (B) Likewise, cells of the proneural clusters of Psn^C1 mutant wing imaginal discs express 22C10 (arrows). By contrast, no expression of 22C10 is detectable in the Su(H)⁴⁷ mutant wing disc (C). (D) Expression of Elav in Psn^C1 mutants. The cells of some clusters, such as the APA+tr1, PPA and PSA clusters, which are fused to one big cluster (arrow), do express Elav. (E) By contrast, Su(H)⁴⁷ mutant wing imaginal discs are devoid of any Elav expression in cells of the notum. The results suggest that the loss of SOP markers in the cells of Su(H) mutant proneural clusters is accompanied by a loss of neural differentiation markers.

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