Fig. 7. Absence of the Brca1 full-length isoform disrupted H2AX localization. Scp3 and H2AX double immunofluorescence localization of control and mutant spermatocytes. (A-D,F-I) Localization of H2AX in unirradiated testes. Localization is similar at the leptotene (Lep) and early zygotene (E. Zyg) stages in control and mutant spermatocytes. Starting from the late zygotene (L. Zyg) stage, H2AX localizes to the XY body in wild-type spermatocytes (arrows, C,D). However, in 90% of spermatocytes from Brca1 mutant mice, H2AX fails to localize to the XY body and remains as multiple foci at the late zygotene stage (H) and early pachytene (E. Pac) stage (I). (E,J) Localization of H2AX in early pachytene stage spermatocytes 10 minutes after 80 Gy irradiation. The XY body cannot be detected.

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