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Fig. 7. A correlation between bandlet slippage and developmental regulation in the
op.pp lineage following ablation of the op.pa cell. The OP lineage was labeled
with rhodamine lineage tracer and the op4.pa cell ablated during
stage 7. The operated embryos were fixed at stage 9 and counterstained with
Hoechst 33258. (A,B) In those embryos in which bandlet slippage did not take
place, the PV and AD clusters are usually missing from the op4
clone. The normal sites of the missing op4 pattern elements are
marked by the white arrowhead and asterisk. (C,D) In embryos that experience
slippage, a gap forms between the portion of the blast cell clone immediately
anterior to the ablated cell and the portion immediately posterior to the
ablated op4.pa cell. Within the gap, no fluorescently labeled cells
are observed, and the ganglia in the gap show a reduced size. A PV cluster
(yellow arrowhead) and an AD cluster (yellow asterisk) are seen immediately
posterior to the gap, and are therefore likely to be derived by regulation of
the op4.pp cell. Orientation of the specimen is the same as in
Fig. 2. Yellow, op4
descendants; light blue, op3 descendants; green, the descendants of
the anteriormost o and p blast cells. Arrowhead indicates PV cluster; asterisk
indicates AD clusters. See Fig.
3 for labeling of additional pattern elements. Scale bar: 20
µm.
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