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Fig. 7. Relationship between larval and adult ALs in early pupal stages. (A-C)
Images of GAL4-GH146 driving membrane-targeted GFP. Each panel is a
single confocal section through the developing AL stained with anti-GFP
antibody (green), anti-N-cadherin antibody (red) and nc82 (blue). Dotted lines
indicate the larval area of the lobe; solid lines indicate the adult-specific
zone. Notice that, in C4, nc82 is extremely weak in spite of an
increase in laser power from 3% to 10% (A4 and B4,
respectively). (D-F) z projections of confocal images of
GAL4-MJ94 driving mCD8-GFP in larval ORNs. Asterisks
indicate the ORN axons of the antennal nerve entering the larval AL; the arrow
in F indicates the `stump' of remaining ORN axon terminals and the arrowheads
indicate examples of nc82-positive densities that seem to lie along the few
remaining ORN processes. Dotted lines indicate the extent of the larval AL.
Green is mCD8-GFP; (D,F) magenta is nc82; (E) red is N-Cadherin and blue is
nc82.
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