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Fig. 8. Candidate cellular components of the developing adult AL. (A1-A5) Time course of repo-GAL4 driving mCD8-GFP expression, marking glial cell membrane. Each panel is a single optical section through the developing AL. (B1-B3) Comparison of repo-GAL4-driven marker expression, and anti-Repo antibody. Repo-GAL4 strongly labels all glia but has weak background staining in all cells. (B4) Location of Repo-positive nuclei in relation to GH146-positive dendrites. (B5) Triple stain of Repo, Elav and TOTO-3 (a DNA stain, most prominent in nucleoli) showing that Repo- and Elav-stained cells (glia and neurons, respectively) account for all nuclei in the vicinity of the AL. (C1-C5) Time course of GAL4-C155 driven UAS:mCD8-GFP (single sections); C3 and C5 correspond to the green channel of C2 and C4, respectively.





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