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Fig. 1. Spatial and temporal expression pattern of lin-3. (A) Different
extents of 5' noncoding and coding regions of lin-3 were cloned
into a gfp reporter construct as described in the Materials and
methods. Construct 1 begins near the 3' end of the fourth intron,
construct 2 begins from the 3' half of the second lin-3 intron,
and constructs 3 and 4 contain the first intron and the first promoter. The
two arrows indicate lin-3 promoters; blue and red boxes represent two
alternative signal peptides right after translational initiation codons;
purple boxes are exons encoding an EGF domain; and yellow box represents a
domain that exists only in one form of lin-3 and is created by
alternative splicing. The arrow from ACEL (anchor cell-specific enhancer of
lin-3) marks the position of an enhancer element that drives the
expression of lin-3 in the AC. Cells expressing lin-3::gfp
were summarized as –, no expression; and +, expression. (B-M) The
expression of lin-3::gfp in pharynx (pha), spermatheca valve (spv), a
subset of the primary vulval cells (vulF), male tail (F and U cells), and in
the anchor cell (AC) and the pre-anchor (AC)/ventral uterine precursor cells
(VU). (B-D,H-J) Nomarski images; (E-G,K-M) corresponding images of
lin-3::gfp expression.
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