|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
|
Fig. 3. Relationship between cell death and basal lamina degradation. Shelves of
developing palates were put in contact and cultured in the presence of either
a caspase inhibitor (z-VAD) or a metalloproteinase inhibitor (MMI). At the end
of culture (24 hours), cell death (green) and laminin (red) were detected in
the same palate slice by TUNEL and specific immunohistochemistry,
respectively. After 12 hours in culture, control palates show an advanced MEE
cell death and basal lamina degradation. At the end of culture, MEE and basal
lamina completely disappeared. However, z-VAD treatment inhibited cell death
but basal lamina remained intact. Application of 10 µM BB3103 (MMI) did not
alter the apoptotic fate of MEE cells but, as expected, inhibited basal lamina
degradation (arrowhead). These experiments were repeated more than three times
with at least a triplicate for each condition. v, blood vessel. Scale bar: 50
µm.
|
