|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
|
Fig. 7. Myt1 is involved in generation of glucagon and
somatostatin-expressing cells in chicken embryonic endoderm and may be in the
same pathway as Ngn3. (A-C) In normal chicken embryos (A) glucagon
(brown staining, black arrow) and somatostatin-expressing cells (data not
shown) are absent in chicken stomach and duodenum. When Nzf2b was
ectopically expressed in the chicken embryonic gut endoderm around stage 18,
glucagon expression was induced in the stomach (B) and duodenum (C). A small,
yet significant, number of somatostatin-expressing cells were also induced (D,
red arrowhead). (E,F) dnMYT1 inhibits NGN3-mediated induction of
glucagon+ cell formation in chicken gut endoderm. Ngn3 +
Myt1a (E) or Ngn3 + dnMyt1 (F) were co-electroporated into
chicken gut endoderm respectively. Glucagon expression (brown antibody
staining) and Myt1 or dnMyt1 expression (blue, in situ
hybridization) was measured. In E, co-expression of Ngn3 and
Myt1a (data not shown)
(Grapin-Botton et al., 2001)
resulted in induction of glucagon+ cells (brown and blue color
overlap resulting in dark brown/purple). In F, cells that co-express
Ngn3 and dnMyt1 almost never express glucagon (brown and
blue cells do not overlap) suggesting that the presence of dnMYT1 inhibited
the ability of NGN3 to promote the generation of glucagon-expressing cells.
Black dashed line, stomach; green dashed line, duodenum; red dashed line,
pancreas.
|
