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Fig. 1. Organization and expression of the Lac gene, and structure of the Lac protein. (A) The Lac gene is uncovered by Df(2R)CB1 and Df(2R)BSC3, but not by Df(2R)vg135. (B) The Lac gene encompasses around 12 kb and its cDNA is around 2.1 kb. BG1462 is inserted at position 46 of the Lac cDNA, 270 bp before the ATG. BG1462 is late embryonic lethal both when homozygous, and over the deficiencies that uncover the Lac gene, but not over Df(2R)vg135. Lac2 is an imprecise excision of BG1462 that removes the ATG. (C) The Lac protein contains three Ig domains and a C-terminal hydrophobic domain, characteristic of GPI-linked proteins. (D-G) In situ hybridization with an antisense Lac probe does not detect any signal in oocytes (not shown). (D) At stage 5, the Lac transcript is detected in the cellularized blastoderm but it is excluded from the ventral side. (E) At germ band extension, a striped pattern of Lac transcript can be detected in the ectoderm. Later on, the Lac transcript accumulates in some cells of the nervous system (F) and in the tracheal cells (G).





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