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Fig. 2. Characterization of the tracheal phenotype of Lac mutants. (A-D,H,I) Projections of several confocal sections showing details of 2-3 tracheal metameres of wild type (A,H), Lac² (B) or Lac¹ (C,D,I) mutants at stage 16, stained only with mAb2A12 to show the lumen (red), or with mAb2A12 and an anti-GFP antibody to detect the expression of tauGFP in tracheal cells (green), which highlights cell shape. Note the defects in mAb2A12 accumulation (arrowhead in C), branch sinuosity (arrows in B,C), and branch breaks (arrows in D,I). (E) Lateral view of a stage 15 wild-type embryo stained with mAb2A12. (F) Lateral view of a stage 15 Lac¹ embryo, in which Lac and tauGFP are expressed in the tracheal cells stained with mAb2A12 (black) and anti-GFP (brown). Note the rescue of the tracheal phenotype. (G) Lateral view of a Lac¹/bulb embryo at stage 16 stained with mAb2A12 (black), showing the same tracheal phenotype as Lac¹ or Lac² mutants. (J-O) Details of the dorsal trunk between tracheal metameres 7 and 8 at stage 16 of wild type (J,L,N) and Lac¹ mutants (K,M,O). Embryos are labelled with anti-GFP (J,K) to show the increased length of the tracheal cells (highlighted by expression of tauGFP) and, as a consequence of the dorsal trunk, with a Crb antibody (L,M), and with a GFP antibody (N,O) to detect nodGFP accumulation in tracheal cells.

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