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Fig. 5. Double in situ hybridization with probes against Hyzic, Cnash and
Nowa, in all possible combinations. (A) Hyzic (red) and
Cnash (blue) show no overlap in their expression patterns. (B)
Similarly, Hyzic (red) and Nowa (blue) are expressed in
different subpopulations of nematoblasts. (C) Only the messages of
Cnash (blue) and Nowa (red) show a substantial
co-localization, as demonstrated by the violet cell nests. (D) Quantitative
analysis of the overlapping expression of Cnash and Nowa.
Scale bars: 50 µm (A-C).