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Fig. 6. BrdU/in situ double labeling. (A-D) BrdU pulse labeling combined with in
situ hybridization against Hyzic (A,D), Cnash (B) and
Nowa (C). Gene expression is shown in black, whereas BrdU-positive
nuclei appear in green. (A) A substantial fraction of
Hyzic-expressing cells are BrdU positive. (B) No
Nowa-expressing cell nests are positive for BrdU. (C)
Cnash-expressing cell nests also show no BrdU signal. (D)
Hyzic/BrdU double labeling further reveals that some BrdU-positive
nests do not express Hyzic (arrows), indicating that not all
proliferating cell nests express Hyzic. (E) Quantitative analysis of
Hyzic, Cnash and Nowa expressing clusters of all sizes that
were continuously labelled with BrdU at 0, 12, 24 and 48 hours.
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