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Fig. 11. VCAM1 immunostaining of isolated whole allantoises and allantoic subregions
after 24 hours of culture. Subregions of whole lacZ/+ donor
allantoises were placed into the exocoelomic cavity of wild-type hosts
(A-C,G-I), cultured for 24 hours, exposed to X-gal and VCAM1 immunostained
(brown color). Wild-type allantoic subregions were cultured in isolation
(D-F). Sections were counterstained with nuclear fast red (A-C,G-I), or
hematoxylin (D-F). (A) Donor distal allantoic 2/3 has fused with the host's
allantoic regenerate (al-r) and chorion exhibits VCAM1. (B) Donor proximal
allantoic 1/3 is tethered to the host's allantoic regenerate (al-r) and
exhibits robust VCAM1. (C) Donor proximal allantoic 1/3 is fused with both the
host's yolk sac (ys) and chorion (ch) and exhibits robust VCAM1. (D-F)
Wild-type distal 2/3 (D), wild-type proximal 1/3 (E), and whole wild-type
allantois (F) from headfold (HF)-stage conceptuses were cultured in isolation
for 24 hours; all exhibit robust VCAM1. (G) Donor distal allantoic 1/3
exhibits VCAM1 and is fused with the host's chorion. (H) Donor
proximal/mid-allantoic region (p+m) is free-floating in the host's exocoelom
and exhibited VCAM1 throughout the explant. (I) Donor whole allantois is fused
with the host's chorion and exhibits strong VCAM1. In A-C, stages of
synchronous donor allantoises and host conceptuses before and after culture
are separated by a `/'. In G-I, initial stages of the asynchronous donor
allantois and host conceptus are separated by `/', whereas the number after
`//' indicates the final stage of the host after 24-hour culture. Scale bar in
I: 50 µm (B); 75 µm (D-F); 100 µm (A,C,G-I).
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