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Fig. 1. Identity of the Fugu rubripes Otx2 gene. (A) Comparison of Fugu and mouse Otx2 genes. Filled boxes represent coding exons. Homeobox regions are shown in gray. (B) Comparison of the deduced amino acid sequences of the Fugu Otx2 (FrOtx2), zebrafish Otx2 (DrOtx2), Xenopus Otx2 (XlOtx2) and mouse Otx2 (MmOtx2). Homeodomains highly conserved between all Otx2 genes are shaded. Otx protein sequences were retrieved from the GenBank database. Degenerate PCR primers are indicated by arrows and Otx tail motifs are underlined. (C) Phylogenetic tree of Otx/Crx family proteins. The tree was constructed using the NJ method. Protein sequences were retrieved from the GenBank database. Bf, amphioxus Branchiostoma floridae; Dr, zebrafish Danio rerio; Fr, Fugu rubripes; Hs, Homo sapiens; Lj, Lampetra japonica; Mm, Mus musculus; Pm, Petromyzon marius; Sc, dogfish Scyliorhinus canicula; Xl, Xenopus laevis.

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