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Fig. 12. Identification of conserved sequence elements. (A,B) The 69 kb Fugu genomic sequences and the 1 Mb mouse genomic sequences, which correspond to –500 to +500 kb of the Otx2 locus were examined by Pipmaker analysis. Regions of significant similarity are indicated by horizontal lines. Conserved sequences and the hypothetical coding region are defined by E1-E8 and by a black bar (Q9NX78), respectively. Colored bars denote the genomic fragments that display specific lacZ activity in Fig. 3. (C,D) Lateral views of F11placZ (C) and E6/7dplacZ (D) transgenic embryos at 10.5 dpc following ß-galactosidase staining.





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