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Fig. 2. Expression analysis of the Fotx2 gene in wild-type pufferfish embryos. (A) Expression patterns of the Fotx2 gene analyzed by RTPCR. The ß-actin fragment was amplified as a control for the quality of cDNA. (B-E) Expression patterns of the Fugu genes analyzed by whole-mount in situ hybridization. Pufferfish no tail expression at 44 hpf (B). Fotx2 expression at 44 (C), 54 (D) and 78 hpf (E). MBT, midblastula transition; a, anterior; an, anterior neural tube; e, eye; fb, forebrain; m, mesencephalon; mhb, mid-hindbrain boundary; s, somites; p, posterior; y, yolk.





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