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Fig. 6. Developmental changes in lacZ expression with the F8placZ construct. Lateral (A,C), frontal (B,D,H) and dorsal (K) views of F8placZ transgenic embryos following ß-galactosidase staining. Transverse and sagittal sections of the transgenic embryos at 10.5 (E-G) and 12.5 (I,J) dpc. (A,B) 9.5, (C-G) 10.5, (H-J) 12.5 and (K) 13.5 dpc transgenic embryos. Whole-mount in situ hybridization analysis of Wnt3a mRNA indicates that mouse Wnt3a expression is detected in the prospective dorsal diencephalon and cortical hem (arrowhead; L). cp, choroid plexus; d, diencephalon; he, cortical hem; m, mesencephalon; t, telencephalon.





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