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Fig. 8. Developmental changes in lacZ expression with the F11placZ construct. Lateral (A-C,I) and dorsal (J) views of the transgenic embryos following ß-galactosidase staining. Transverse and sagittal sections of the transgenic embryos at 10.5 (D-G) and 12.5 dpc (H). (A) 9.5, (B, D-G) 10.5, (C) 11.5, (H) 12.5 and (I,J) 13.5 dpc transgenic embryos. (E,F,G) Transverse section through the cephalic region of a 10.5 dpc transgenic embryo, showing lacZ expression in the trigeminal ganglions (V) and the ventral portion of the spinal cord (arrowheads). (K) Whole-mount in situ hybridization analysis of mouse Otx2 expression at 9.5 dpc. Otx2 expression is also detected in the trigeminal nerves and the first branchial groove (arrowheads). d, diencephalon; m, mesencephalon; p, p1 diencephalon; t, telencephalon; V1, opthalmic branch of the trigeminal nerve; V2, maxillar branch of the trigeminal nerve.





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