|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
|
Fig. 1. sotv and botv are novel Drosophila EXT genes.
All clones are marked by shavenoid– in
Drosophila adult wing blade. (A) Wild-type wing. Longitudinal veins 1
to 5 (L1 to L5) are shown. (B) ttv mutant clone made between L1 and
L2. The tissues within the clone are deleted and wing blade becomes narrower
in A/P orientation. (C) sotv mutant clones. Note that veins L3 and L4
are abnormally close to each other. The region between L3 and L4 is patterned
by Hh, whereas the rest of the wing blade is patterned by Dpp. sotv
phenotype is relatively weaker than other EXT mutants ttv and
botv. (D,E) botv mutant clones located between L2 and L3 or
behind L4. The tissues within the clone are deleted. (F) Structures of
Drosophila EXTs, Ttv, Sotv and Botv deduced from genome sequence.
These proteins have three domains as indicated: light green, transmembrane
region; light blue, putative catalytic region; blue, DXD motif. Amino acid
sequence alterations in Ttv, Sotv and Botv caused by EMS mutation are
indicated by arrows. (G) Glycosaminoglycan synthesis in Drosophila
(from Toyoda et al., with modification)
(Toyoda et al., 2000). (H)
Sequence alignment of Ttv, Sotv and Botv proteins. Black boxes indicate amino
acids that are identical in these proteins. The overall similarity between
them is 26-32%. The homology is especially high in their C-terminal regions.
Conserved DXD motif is indicated in red. Gal, galactose; GlcA, glucuronic
acid; GlcNAc, N-acetylglucosamine; Xyl, xylose.
|
