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Fig. 7. Morphogens are accumulated in front of the mutant clones. (A-I) Hh diffusion patterns in the wild-type disc (A-C) and disc with ttv⁵²⁴botv⁵¹⁰ clone (D-I). The staining intensity of Hh in the selected area (white boxes in B,E,H) was integrated along the A-P axis, plotted using NIH Image software and presented schematically (C,F,I). (A-C) In the wild-type disc, Hh flows into the anterior compartment with a moderate gradient starting from the middle of the posterior compartment. The distribution pattern of Hh in the ventral region (upper graph in C) is similar to that in the dorsal region (lower graph in C). (D-F) When the mutant clone was located in the anterior-ventral region along the A-P boundary, Hh was accumulated in the posterior-ventral cells. Compare the levels of Hh at the A-P boundary in the ventral region with that in the dorsal region (graphs in F). (G-I) Likewise, when the EXT clone was in the anterior-dorsal compartment, Hh accumulated in the posterior-dorsal cells (graphs in I). (J,K) Dpp-GFP accumulated in front of the EXT mutant clone. In the ventral compartment, Dpp-GFP diffuses with moderate gradient (upper bracket in J). Dpp-GFP accumulated on the cell surface of the wild-type cells adjacent to the mutant clone (lower bracket in J). Compare the levels of Dpp-GFP in the region with mutant clone (lower arrowhead in J) with that in the region without clone (upper arrowhead in J). (L,M) Wg accumulated in front of the EXT mutant clone. Compare the levels of Wg in the region with mutant clone (left bracket and arrowhead in L) with that in the region without clone (right bracket and arrowhead in L). For Hh and Wg, the same results were obtained in cells mutant singly for any of the EXT genes. Single mutation was not tested for the distribution of Dpp-GFP.

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