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Fig. 6. NOS-3 is required for fbf-1 fbf-2 double mutant Glp phenotype. (A)
fbf-1(ok91) fbf-2(q704) and fbf-1(ok91) fbf-2(q704)
nos-3(oz231) animals one day past L4 were dissected and stained with
REC-8 (proliferative) and HIM-3 (meiotic) antibodies
(Hansen et al., 2004). The
graph shows the average number of cell diameters along the length of the gonad
arm that cells are proliferative (REC-8, green) or meiotic (HIM-3, red). The
proliferative zones of 8/10 fbf-1(ok91) fbf-2(q704) nos-3(oz231) arms
were smaller or of similar size to those of wild-type, while 2/10 were much
larger (33 and 40 cell diameters). The phenotype is independent of germline
sex as fog-3(q443); fbf-1(ok91) fbf-2(q704); unc-32(e189) and
fog-3(q443); fbf-1(ok91) fbf-2(q704) nos-3(oz231); unc-32(e189)
animals were similar to the unfeminized animals (data not shown). Error bars =
1 s.d. (B) Dissected gonad arm of fog-3(q443); fbf-1(ok91) fbf-2(q704)
nos-3(oz231); unc-32(e189) young adult animal stained with DAPI (blue),
REC-8 (green) and GLD-1 (red). Distal is to the left. Scale bar: 20 µm. (C)
Dissected gonad arms of wild-type (top) and gld-2(q497) fog-3(q443);
fbf-1(ok91) fbf-2(q704) nos-3(oz231); unc-32(e189) (bottom) stained with
DAPI (blue) and GLD-1 (red). Only a portion of the distal arms are shown with
distal to the left.
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