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Fig. 3. Expression of laminin chains in normal and mutant early postimplantation
embryos. (A-G) Arrowheads indicate the embryonic basement membrane and the
arrow indicates RM. (A) At E5.5, laminin 
1 was detected in the
embryonic basement membrane and in RM. (B) At E6.5, laminin 5 (red) is
detected primarily in the embryonic basement membrane, though some is apparent
in RM at the ectoplacental pole (asterisks). Laminin 1 (green) is
abundant in RM, but its presence in the embryonic basement membrane is
obscured by the 5 staining. (C,E-G) At E7.5 in wild type, laminin
1 (green) is present in both the embryonic basement membrane and in RM,
but laminin 5 (red) appears confined primarily to the embryonic
basement membrane. (D) This embryo is Lama5-/-,
demonstrating the specificity of the 5 antibody. Note staining for
5 in the surrounding maternal tissue. (F) Note the absence of 5
in RM. (G) Nuclei (blue) are visible in the merged images. (H-J) X-gal
staining of sections of Lama1+/- and
Lama1-/- embryos, as indicated. Cells of the PE at the
periphery of the normal embryos stain blue (H,I). In the mutant, the cells
stain blue but are found in a cluster next to the small embryo (asterisk in J)
owing to failure to migrate. Scale bar in A: 100 µm for A-D,H-J; 33.3 µm
for E-G.
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