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Fig. 6. Analysis of basement membranes in Lama1-/-; Mr5
embryos. (A-D) Embryos were double stained for laminin 
1 (green) and
laminin 5 (red). Arrowheads indicate the embryonic basement membrane,
and arrows indicate RM, when present. (A,B) At E6.5, the
Lama1-/-; Mr5 embryo (B) has laminin 5 in the
embryonic basement membrane but lacks laminin 1 and RM, when compared
with the control (A). (C,D) At E7.5, Lama1-/-; Mr5 embryos
appear to still be alive and to have an embryonic basement membrane containing
laminin 5. (E,F) Embryos were triple stained for laminin ß1 (red),
cytokeratin 8 (green) and nuclei (blue). The Lama1-/-; Mr5
embryo is much smaller than the control, but both contain embryonic ectoderm
(EE), extra-embryonic ectoderm (ExE) and ectoplacental cone (EPC). Trophoblast
blood sinuses (S) are present in the control but are lacking in the
Lama1-/-; Mr5 embryo. Scale bars: in D, 100 µm for A-D;
in F, 100 µm for E,F.
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