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Fig. 1. Asymmetric histone H3 methylation of lysine 9 in the parental genomes of
one-cell mouse embryos. Mouse GV-stage (GV) and MII-stage (MII) oocytes,
one-cell embryos collected 3, 6 and 12 hours after in vitro fertilization (IVF
3, 6, 12 h) (A), and parthenogenetic and androgenetic one-cell embryos (B),
were subjected to immunocytochemistry with antibody to methylated histone H3
Lys 9 (MeH3/K9). The antibody was localized with FITC-conjugated secondary
antibodies (green), and DNA was stained with DAPI (blue). The parthenogenetic
embryos were prepared by exposing MII-stage oocytes to 7% ethanol for 6
minutes, and collected for immunocytochemistry 6 hours later. The androgenetic
embryos were prepared by fertilizing enucleated oocytes in vitro, and
collected for immunocytochemistry 6 hours after fertilization. Arrows indicate
the male (m) and female (f) pronuclei. pb, polar body. The experiments were
conducted at least three times and similar results were obtained. More than 25
images were obtained for each type of embryo and representative examples are
shown.
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