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Fig. 6. Involvement of protein synthesis and gene expression in H3/K9 methylation
in mouse embryos. (A) Cycloheximide (50 µg/ml) was added at the time of
insemination, and H3/K9 methylation (MeH3K9) was examined 12 hours
post-insemination. (B) 
-Amanitin (25 µg/ml) was added 4 hours after
activation of the androgenetic embryos, and H3/K9 methylation was examined 32
hours post-activation. The polyclonal antibody that recognizes methylated
H3/K9 was localized with FITC-conjugated secondary antibodies (green), and the
DNA was stained with DAPI (blue). The experiments using cycloheximide and
-amanitin were conducted three and two times, respectively, and similar
results were obtained. 21, 12, 18 and 11 embryos were examined in the
cycloheximide (+), cycloheximide (-), -amanitin (+) and
-amanitin (-) groups, respectively, and representative examples are
shown.
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