|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
|
Fig. 3. dm4 mutants have reduced nuclear size. (A) DAPI stained
fat body (arrows) and salivary glands (arrowheads) from
dm4/Y hemizygous mutants or control
dm1-14-2/Y hemizygous males, harvested at the indicated
times AED. Lower magnification (72 hr-low mag) shows the difference in
salivary gland size by 72 hours AED. (B-I) Control
dm1-24-2/Y (B,C,F,G) and dm4/Y
(D,E,H,I) larvae were fed BrdU from 30 to 48 hours AED. Midgut (B-E) and fat
body (F-I) were stained with anti-BrdU (B,D,F,H) and DNA was visualized with
propidium iodide (C,E,G,I). (J) The percentage of BrdU-positive cells in
control (white bars) or dm4/Y (black bars) fat body and
midgut tissue was determined for the experiment above. Fifteen to 21 tissue
samples were analyzed for each bar of the graph, each consisting of an average
of 
50 cells.
|
