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Fig. 4. dMyc promotes and dMnt blocks endoreplication. Flp/Gal4 was used to
clonally express dMyc (A-C,G-K) or dMnt (D-F) with GFP (green). DNA is stained
with DAPI (blue). (A-F) Heat shock-induced clones in fat body from third
instar larvae; DNA and GFP images are merged in C and F. (G) Quantification of
nuclear DAPI fluorescence. DAPI stained fat body nuclei from larvae of the
indicated ages were analyzed by deconvolution microscopy and heat
shock-independent GFP marked dMyc-expressing cells were compared with
neighboring non-GFP control cells. (H-K) Fat body from a second instar larva
containing a heat shock-independent dMyc expressing FLP/Gal4 clone [marked
with GFP (I) and outlined in white] was stained with DAPI (H) and
anti-fibrillarin (J). DAPI and anti-fibrillarin images are merged in K.
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