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Fig. 4. Effects exerted by ectopic expression of FoxA4a/Pintallavis and
Xvent2, and repression and activation constructs on the expression of
endogenous Xanf1. All embryos were microinjected with a mixture of
FLD and mRNA. (AD) Ectopic expression of EnRFoxA4a/Pintallavis and
EnRXvent fusions elicit inhibition of Xanf1 expression.
Microinjection of mRNA: FoxA4a/Pintallavis (A,A');
Xvent2 (B,B'); EnR FoxA4a/Pintallavis (C,C');
EnRXvent2 (D,D'). (B1,B1',B2,B2') Representative
histological sections at two sagittal levels (1 and 2) of the embryo shown in
B and B' demonstrate that descendants of the microinjected blastomeres
labelled with FLD are primarily localised in the neurectoderm. Only single
labelled cells are found in underlying endomesoderm. (E-G) Ectopic expression
of dominant-negative versions of FoxA4a/Pintallavis and
Xvent2 result in posterior expansion of endogenous Xanf1
expression. Red and black arrows indicate position of the posterior border of
the Xanf1 expression domain within microinjected and
non-microinjected (normal) zones, respectively. Microinjection of mRNA:
VP-16-FoxA4a/Pintallavis (E,E'); VP16-Xvent2
(F,F'); Xvent2-P40 (G,G'). (E1,E1') Representative
histological section at level 1 of the embryo shown in E and E'
illustrates that microinjected blastomeres primarly populate the neurectoderm
and that endogenous Xanf1 is now expressed in these cells.
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