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Fig. 3. Su(H) is required for Notch to repress Twist. (A,C,E,G,I) Lateral views of stage 10 whole-mount embryos stained with anti-Twist. (B,D,F,H,J) Corresponding close-ups of embryos in A,C,E,G,I. (A,B) Su(H)^null; UAS-N^intra embryo has a wild-type-like Twist pattern, similar to Su(H)^null. Low and high Twist domains are seen along the anterior-posterior axis; Twist is not strongly repressed as in UAS-N^intra embryos (see Fig. 1). (C,D) N^null; UAS-FLN embryo exhibits low and high Twist domains. FLN rescues the Twist pattern of N^null embryos (see Fig. 1). (E,F) N^null; UAS-N^intra embryo has low and high Twist domains. N^intra rescues the Twist pattern, but represses Twist expression compared to FLN. (G,H) N^null; UAS-FLNcdc10 maintains uniform high Twist expression, similar to N^null embryos. FLNcdc10 does not rescue the Twist pattern. (I,J) N^null; UAS-Su(H)-VP16 embryo no longer maintains uniform high Twist expression. Low and high Twist domains are observed, but high Twist domains appear slightly expanded. Black arrowhead points to cells in the low Twist domain that express higher levels of Twist than wild type. Su(H)-VP16 rescues the Twist pattern but not as strongly as FLN and N^intra.

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