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Fig. 1. Phylogenetic analysis of T-box genes. (A) Phylogenetic tree. T-box domain
amino acid sequences of all C. elegans T-box genes and representative
members of the defined T-box subfamilies found in other organisms
(Papaioannou, 2001) were
aligned using ClustalW accessed via the European Bioinformatics Institute
(EBI,
http://www.ebi.ac.uk/).
The alignment was subjected to Phylip analysis using the same EBI interface
and Phylip outputs were interpreted using the tree drawing programme TreeView.
Non-C. elegans genes are in red. Species abbreviations are as
follows: As, ascidian; Dm, Drosophila melanogaster; Hs,
Homo sapiens; Mm, Mus musculus. New C. elegans gene
names (tbx-30-41) have been approved by the Caenorhabditis Genetics
Centre (CGC). (B) Genomic organisation of tbx-8 and tbx-9
(and neighbouring genes) in C. elegans and C. briggsae.
Ce-tbx-8 and Ce-tbx-9 are transcribed in opposing directions
(arrows), whereas Cb-tbx-8 and Cb-tbx-9 are transcribed in
the same direction (arrows). The thick dotted line represents the intergenic
region. Ce-tbx-8 and Cb-tbx-8 are 73% identical throughout
their T-box domains. Ce-tbx-9 and Cb-tbx-9 are 56%
identical. There is evidence of a local chromosome inversion in C.
briggsae compared with C. elegans, which extends for several
genes to the right of Cb-tbx-9 (neighbouring region not drawn to
scale). Red bar denotes the T-box domain of Ce-tbx-8 and
Ce-tbx-9 (59% identical).
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