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Fig. 3. Hypodermal defects in tbx-8/tbx-9(RNAi) animals. The arrangement
of hypodermal cells in embryos and L1 larvae has been visualised using the
ajm-1::GFP reporter. (A-D) Dorsal view. (A) Wild-type embryo around
300 minutes following first cleavage. The two rows of dorsal hypodermal cells
born 1 hour previously have intercalated into a single row and their advancing
edges have come into contact with the opposing lateral hypodermal cells
(arrows). (B-D) tbx-8/tbx-9(RNAi) embryos at approximately the same
stage. Dorsal hypodermal cells are clearly disorganised. In most embryos
dorsal intercalation begins relatively normally at the anterior (B-D, arrow)
but does not proceed normally in the midregion or posterior (B-D, arrowheads).
In these embryos, dorsal intercalating cells that are not the appropriate
wedge-shape can be seen (B-D, asterisks), and many cells do not extend
contralaterally in the correct way. Dorsal intercalation often arrests at this
point, the same misarranged cells being observable at least 1 hour later.
(E-H) Arrangement of lateral hypodermal (seam) cells in
tbx-8/tbx-9(RNAi) embryos and L1 larvae. (E) Wild-type embryo
beginning elongation, lateral view. A linear row of seam cells can be seen.
(F) tbx-8/tbx-9(RNAi) embryo, same stage and view. The lateral row of
cells is interrupted, with some cells being pinched out of line (asterisks).
(G) tbx-8/tbx-9(RNAi) animal that has survived to hatching; some seam
cells are pinched out of line (asterisks) and are misshapen at the site of the
dorsal bulge (arrow). Animals are much shorter than they should be in L1
(compare with (H) wild type, posterior half only of animal, same scale).
Posterior is to the right in all panels. Scale bars, 10 µm.
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