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Fig. 6. vab-7 expression in tbx-8/tbx-9(RNAi) embryos. (A-C)
Similarity of expression domains of tbx-8 (A), tbx-9 (B) and
vab-7 (C) GFP reporter constructs around the 400-cell stage.
In all three cases, expression is seen in posterior muscle cells, although the
domain of tbx-8 expression is slightly wider, with some
non-vab-7-expressing nuclei showing up (A, arrowheads). (D)
vab-7::GFP expressing nuclei at the 1.5-fold stage.
Expression is seen in posterior muscle cells (arrowheads), the majority of
which are dorsal, and in the posterior hypodermal cells hyp 8-11 (arrow, 4-5
nuclei). The vab-7::GFP construct gives a similar expression pattern
to the vab-7::lacZ construct and antibody staining previously
reported (Esmaeili et al.,
2002; Ahringer,
1996). (E) vab-7::GFP expression in
tbx-8/tbx-9(RNAi) embryos at the same stage. No muscle-specific
expression of vab-7 is observed, even though the same number of
muscle cells are present (see text). vab-7 expression is still seen
in one or two posterior hypodermal cells (arrow), which appear to have shifted
slightly anteriorly, presumably due to the general disorganisation of
tbx-8/tbx-9(RNAi) embryos. Posterior is to the right. Scale bar, 10
µm.
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