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Fig. 1. Dorsal binding clusters identify regulatory DNAs. Diagrams on the left show
the locations and sizes of five Dorsal binding clusters (depicted as blue
boxes with sizes indicated below) identified in an earlier study
(Markstein et al., 2002). In
situ hybridization assays were performed to identify the expression profiles
of the protein-coding genes (indicated as green boxes) located near the
different clusters. Those genes found to be differentially expressed along the
dorsal-ventral axis are shown in the middle column (`endogenous expression').
Genomic DNA fragments that encompass each of the five Dorsal-binding clusters
were fused with a eve-lacZ reporter gene and expressed in transgenic
embryos. Reporter gene expression (right column) was visualized by in situ
hybridization using a digoxigenin-labeled lacZ antisense RNA probe.
There is a close correspondence between the expression patterns of the
endogenous genes and the staining patterns obtained with the fusion genes:
sog (A) and CG12443 (B) are expressed throughout the
neurogenic ectoderm; brk (C) is expressed in the ventral neurogenic
ectoderm; and Phm (D) and Ady43A (E) are expressed in the
mesoderm. Lateral views of cellularizing embryos oriented with anterior to the
left and dorsal up are shown.
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