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Fig. 10. Hh internalization in Hh-receiving cells in the absence of Ptc. (A) ptc¹⁴ clone (labeled by the lack of ß-gal staining, blue) abutting the AP compartment border in an hh-Gal4/UAS-HhGFP background stained with anti-Ptc antibody (red). (a) Magnification of the same ptc¹⁴ clone in A. There is no colocalization between HhGFP punctuate structures and Ptc¹⁴ (arrowheads). Only a few of the Hh-GFP vesicles show colocalization with Ptc staining, though not as spots (circles). Compare with HhGFP staining in a wild-type background (Fig. 1D). (B) ptc¹⁴ clone in an hh-Gal4/UAS-HhGFP wing disc with internalized Red-dextran. (b) Magnification of the ptc¹⁴ clone in B. HhGFP vesicles do not colocalize with Ptc but most of them colocalize with internalized Red-dextran (circles). (C) ptc¹⁶ clone (indicated by the lack of ß-gal staining, blue) abutting the AP compartment border in an hh-Gal4/UASHhGFP background stained with anti-Ptc antibody (red). (c) Magnification of the ptc¹⁶ clone in C. (D) ptc¹⁶ clone in an hh-Gal4/UAS-HhGFP wing disc showing the internalized Red-dextran. (d) Magnification of the ptc¹⁶ clone in D. Observe the HhGFP punctuate structures (green, arrowheads) in A cells close to the AP border with no Ptc protein (c). These structures colocalize with Red-dextran (circles; d). Scale bars: 50 µm in A-D; 15 µm in a-d.

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