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Fig. 2. The Ptc^WTGFP chimera behaves as the wild-type Ptc protein. (A) Scheme of the PtcGFP fusion protein. The GFP is tagged to the C-terminal cytoplasmic tail of Ptc. (B) Western blot of protein extracts from AB1-Gal4/UAS-Ptc^WTGFP or AB1-Gal4/UAS-Ptc¹⁴GFP salivary glands stained with anti-GFP and anti-Tubulin. Both Ptc^WTGFP and Ptc¹⁴GFP proteins migrate with an apparent molecular weight of 180-200 kDa. (C) Collier (Col) expression pattern in a wild-type wing imaginal disc. (D) A large ectopic Ptc^WTGFP clone in the P compartment causes a reduction of Col expression (arrowhead) at the AP compartment border because of the sequestration of Hh by the ectopic Ptc (see also Fig. 7A). (E) An ectopic Ptc^WTGFP clone in the P compartment of a wing imaginal disc stained with anti-Smo (red). Note the autonomous downregulation of Smo because of the repressive activity of Ptc (arrowheads).

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